diff --git a/components/form/__tests__/__snapshots__/demo.test.js.snap b/components/form/__tests__/__snapshots__/demo.test.js.snap
index 682d335122..498e70f8d5 100644
--- a/components/form/__tests__/__snapshots__/demo.test.js.snap
+++ b/components/form/__tests__/__snapshots__/demo.test.js.snap
@@ -471,6 +471,137 @@ exports[`renders ./components/form/demo/basic.md correctly 1`] = `
 </form>
 `;
 
+exports[`renders ./components/form/demo/col-24-debug.md correctly 1`] = `
+<form
+  autocomplete="off"
+  class="ant-form ant-form-horizontal"
+  id="basic"
+>
+  <div
+    class="ant-row ant-form-item"
+  >
+    <div
+      class="ant-col ant-col-24 ant-form-item-label"
+    >
+      <label
+        class="ant-form-item-required"
+        for="basic_username"
+        title="Username"
+      >
+        Username
+      </label>
+    </div>
+    <div
+      class="ant-col ant-col-24 ant-form-item-control"
+    >
+      <div
+        class="ant-form-item-control-input"
+      >
+        <div
+          class="ant-form-item-control-input-content"
+        >
+          <input
+            class="ant-input"
+            id="basic_username"
+            type="text"
+            value=""
+          />
+        </div>
+      </div>
+    </div>
+  </div>
+  <div
+    class="ant-row ant-form-item"
+  >
+    <div
+      class="ant-col ant-col-24 ant-form-item-label"
+    >
+      <label
+        class="ant-form-item-required"
+        for="basic_password"
+        title="Password"
+      >
+        Password
+      </label>
+    </div>
+    <div
+      class="ant-col ant-col-24 ant-form-item-control"
+    >
+      <div
+        class="ant-form-item-control-input"
+      >
+        <div
+          class="ant-form-item-control-input-content"
+        >
+          <span
+            class="ant-input-affix-wrapper ant-input-password"
+          >
+            <input
+              action="click"
+              class="ant-input"
+              id="basic_password"
+              type="password"
+              value=""
+            />
+            <span
+              class="ant-input-suffix"
+            >
+              <span
+                aria-label="eye-invisible"
+                class="anticon anticon-eye-invisible ant-input-password-icon"
+                role="img"
+                tabindex="-1"
+              >
+                <svg
+                  aria-hidden="true"
+                  data-icon="eye-invisible"
+                  fill="currentColor"
+                  focusable="false"
+                  height="1em"
+                  viewBox="64 64 896 896"
+                  width="1em"
+                >
+                  <path
+                    d="M942.2 486.2Q889.47 375.11 816.7 305l-50.88 50.88C807.31 395.53 843.45 447.4 874.7 512 791.5 684.2 673.4 766 512 766q-72.67 0-133.87-22.38L323 798.75Q408 838 512 838q288.3 0 430.2-300.3a60.29 60.29 0 000-51.5zm-63.57-320.64L836 122.88a8 8 0 00-11.32 0L715.31 232.2Q624.86 186 512 186q-288.3 0-430.2 300.3a60.3 60.3 0 000 51.5q56.69 119.4 136.5 191.41L112.48 835a8 8 0 000 11.31L155.17 889a8 8 0 0011.31 0l712.15-712.12a8 8 0 000-11.32zM149.3 512C232.6 339.8 350.7 258 512 258c54.54 0 104.13 9.36 149.12 28.39l-70.3 70.3a176 176 0 00-238.13 238.13l-83.42 83.42C223.1 637.49 183.3 582.28 149.3 512zm246.7 0a112.11 112.11 0 01146.2-106.69L401.31 546.2A112 112 0 01396 512z"
+                  />
+                  <path
+                    d="M508 624c-3.46 0-6.87-.16-10.25-.47l-52.82 52.82a176.09 176.09 0 00227.42-227.42l-52.82 52.82c.31 3.38.47 6.79.47 10.25a111.94 111.94 0 01-112 112z"
+                  />
+                </svg>
+              </span>
+            </span>
+          </span>
+        </div>
+      </div>
+    </div>
+  </div>
+  <div
+    class="ant-row ant-form-item"
+  >
+    <div
+      class="ant-col ant-col-24 ant-form-item-control"
+    >
+      <div
+        class="ant-form-item-control-input"
+      >
+        <div
+          class="ant-form-item-control-input-content"
+        >
+          <button
+            class="ant-btn ant-btn-primary"
+            type="submit"
+          >
+            <span>
+              Submit
+            </span>
+          </button>
+        </div>
+      </div>
+    </div>
+  </div>
+</form>
+`;
+
 exports[`renders ./components/form/demo/control-hooks.md correctly 1`] = `
 <form
   class="ant-form ant-form-horizontal"
diff --git a/components/form/demo/col-24-debug.md b/components/form/demo/col-24-debug.md
new file mode 100644
index 0000000000..5524eb3092
--- /dev/null
+++ b/components/form/demo/col-24-debug.md
@@ -0,0 +1,65 @@
+---
+order: 9999
+title:
+  zh-CN: 测试特殊 col 24 用法
+  en-US: Test col 24 usage
+debug: true
+---
+
+## zh-CN
+
+See issue [#32980](https://github.com/ant-design/ant-design/issues/32980).
+
+## en-US
+
+See issue [#32980](https://github.com/ant-design/ant-design/issues/32980).
+
+```tsx
+import { Form, Input, Button } from 'antd';
+
+const Demo = () => {
+  const onFinish = (values: any) => {
+    console.log('Success:', values);
+  };
+
+  const onFinishFailed = (errorInfo: any) => {
+    console.log('Failed:', errorInfo);
+  };
+
+  return (
+    <Form
+      name="basic"
+      labelCol={{ span: 24 }}
+      wrapperCol={{ span: 24 }}
+      initialValues={{ remember: true }}
+      onFinish={onFinish}
+      onFinishFailed={onFinishFailed}
+      autoComplete="off"
+    >
+      <Form.Item
+        label="Username"
+        name="username"
+        rules={[{ required: true, message: 'Please input your username!' }]}
+      >
+        <Input />
+      </Form.Item>
+
+      <Form.Item
+        label="Password"
+        name="password"
+        rules={[{ required: true, message: 'Please input your password!' }]}
+      >
+        <Input.Password />
+      </Form.Item>
+
+      <Form.Item>
+        <Button type="primary" htmlType="submit">
+          Submit
+        </Button>
+      </Form.Item>
+    </Form>
+  );
+};
+
+ReactDOM.render(<Demo />, mountNode);
+```
diff --git a/components/form/style/horizontal.less b/components/form/style/horizontal.less
index d498a49db2..f8238982ae 100644
--- a/components/form/style/horizontal.less
+++ b/components/form/style/horizontal.less
@@ -9,6 +9,9 @@
   }
   .@{form-item-prefix-cls}-control {
     flex: 1 1 0;
+  }
+  // https://github.com/ant-design/ant-design/issues/32980
+  .@{form-item-prefix-cls}-control:not(.@{ant-prefix}-col) {
     // https://github.com/ant-design/ant-design/issues/32777
     min-width: 0;
   }